Biological markers to establish a relationship between the health status of the St. Lawrence River yellow perch (Perca flavescens) with a gradient of anthropogenic disturbances

• Published in: The Science of the total environment Vol. 726; p. 138515
• Main Authors: Landry, C., Houde, M., Brodeur, P., Boily, M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.07.2020
• Subjects: AChE; Biomarkers; Discriminant analysis; Lycopene; Pesticides; Retinoids; Tocopherol; AChE; Discriminant analysis; Pesticides; Biomarkers; Retinoids; Tocopherol; Lycopene
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.138515

Since the mid-1990s, the decline of the yellow perch population of Lake Saint-Pierre (hereinafter LSP) in Quebec, Canada has been the subject of several research programs. The combined effect of habitat deterioration, the presence of invasive species, and poor water quality negatively affected the yellow perch population in this lake. In 2013, we sampled yellow perch (larvae, juveniles and adults) at six sites along the St. Lawrence River representing a gradient of increasing human influences from upstream to downstream and measured several biomarkers including retinoid compounds (vitamin A). In the most contaminated sites (LSP, north and south shores), we found that retinoid stores were decreased in all three stages of development. To corroborate these results and to test other biomarkers, we once again sampled yellow perch (adults only) from the same sites. Results from our 2014 and 2015 samplings confirmed that LSP yellow perch appeared to be at a disadvantage compared to fish from upstream populations. Individuals from LSP have lower acetylcholinesterase (AChE) activity as well as lower retinoid levels in liver and plasma. These fish were also marked by lower levels of antioxidants such as lycopene and vitamin E. A discriminant analysis of this set of results confirmed that the yellow perch of the LSP could be easily discriminated from those of the other sites (2014 and 2015) on the basis of liver retinoid and, to a lesser extent, of the liver tocopherol and protein concentration of the muscle, as well as AChE activity and DROH (all-trans-3,4-dehydroretinol) measured in plasma. [Display omitted] •Multiple biomarkers tested in adult yellow perch in St. Lawrence River, Québec, Canada.•Fish from contaminated sites had lower AChE activity and antioxidants lycopene and tocopherol.•For 2 consecutive years, retinoid compounds were depleted in yellow perch from the most contaminated sites.•Retinoid-based biomarkers were relevant to distinguish fish from the most contaminated sites.