Human Herpesvirus 8 and Epstein Barr-Virus in a Cutaneous B-Cell Lymphoma and a Malignant Cell Line Established from the Blood of an AIDS Patient

• Published in: Leukemia & lymphoma Vol. 35; no. 3-4; pp. 379 - 387
• Main Authors: Morand, Patrice, Buisson, Marlyse, Collandre, Helene, Chanzy, Bruno, Genoulaz, Odile, Bourgeat, Marie-Joseite, Pinel, Nicole, Leclercq, Pascale, Leroux, Dominique, Marechal, Vincent, Fritsch, Laurent, Ruigrok, Rob, Seigneurin, Jean-Marie
• Format: Journal Article
• Language: English
• Published: United States Informa UK Ltd 1999; Taylor & Francis
• Subjects: Acquired Immunodeficiency Syndrome - blood; Acquired Immunodeficiency Syndrome - complications; Acquired Immunodeficiency Syndrome - pathology; Acquired Immunodeficiency Syndrome - virology; AIDS/HIV; cell line; Epstein-Barr virus; Herpesvirus 4, Human - isolation & purification; Herpesvirus 8, Human - isolation & purification; human herpesvirus 8; Humans; Lymphoma; Lymphoma - etiology; Lymphoma - virology; Lymphoma, B-Cell - etiology; Lymphoma, B-Cell - virology; Molecular Sequence Data; Skin Neoplasms - etiology; Skin Neoplasms - virology; Tumor Cells, Cultured
• ISSN: 1042-8194; 1029-2403
• DOI: 10.3109/10428199909145743

Human Herpesvirus 8 (HHV-8) has been consistently associated with Primary Effusion Lymphoma (PEL or body-cavity-based lymphoma) but not with other lymphomas. This paper reports on an AIDS patient without obvious malignant effusion in body cavities but with a cutaneous lymphoma where HHV-8 and Epstein-Barr virus (EB V) were detected by PCR and electron microscopy. Both viruses were also detected in all the cells of a malignant cell line (BBG1) established from the patient's peripheral blood mononuclear cells. As in PEL and PEL-derived cell lines, both the tumor and the lines lacked B-antigen expression in immuno-logical studies but were of the same B origin as shown by clonal immunoglobulin gene rearrangements. In contrast to other co-infected cell lines, BBGl and subclones spontaneously expressed the HHV-8 lytic antigens p40, p27, p60 and the EBV transfonning latent antigen EBNA2. These data suggest that the clinical and biological features of HHV-8-and EBV-asso-ciated lymphomas could be wider than has been described to date in PEL particularly with the in vivo presence of circulating malignant dually-infected cells engaged in a spontaneous HBV-8lytic infection.