Human carbonic anhydrase VII protects cells from oxidative damage

Human carbonic anhydrase (hCA) VII is a cytosolic enzyme with high carbon dioxide hydration activity. Recently, -glutathionylation of two cysteine residues from the enzyme was revealed, suggesting a new role as oxygen radical scavenger. We analyzed the effect of native and tetramutated hCA VII (all...

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Published inBiological chemistry Vol. 394; no. 10; pp. 1343 - 1348
Main Authors Del Giudice, Rita, Monti, Daria M., Truppo, Emanuela, Arciello, Angela, Supuran, Claudiu T., De Simone, Giuseppina, Monti, Simona M.
Format Journal Article
LanguageEnglish
Published Germany De Gruyter 01.10.2013
Subjects
Blotting, Western
carbonic anhydrase
Carbonic Anhydrases - metabolism
eukaryotic cells
Fluorescent Antibody Technique
glutathionylation
HeLa Cells
Humans
Oxidative Stress - physiology
oxidative stress protection
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Summary:Human carbonic anhydrase (hCA) VII is a cytosolic enzyme with high carbon dioxide hydration activity. Recently, -glutathionylation of two cysteine residues from the enzyme was revealed, suggesting a new role as oxygen radical scavenger. We analyzed the effect of native and tetramutated hCA VII (all cysteines mutated into serines) in a eukaryotic system by stressing cells with an oxidant agent. Results clearly show that native hCA VII can protect cells from oxidative damage by preventing the apoptosis cascade and that cysteines play a leading role in this process. Our findings definitively confirm hCA VII protective role toward oxidative insult.
Bibliography:ObjectType-Article-1
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ISSN:1431-6730
1437-4315
DOI:10.1515/hsz-2013-0204