Inner Structure of Intact Chloroplasts Observed by a Low Temperature Laser Scanning Microscope

Inner structure of isolated intact chloroplasts was observed for the first time by a method of laser scanning microscopy at the temperature of liquid nitrogen at 77 K. The microscope, based on gradient index optics, has a maximum resolution of 440 nm at the wavelength of 650 nm. Chloroplasts were ex...

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Bibliographic Details
Published inPhotosynthetica Vol. 38; no. 4; pp. 493 - 496
Main Authors Vácha, F, Vácha, M, Bumba, L, Hashizume, K, Tani, T
Format Journal Article
LanguageEnglish
Published Springer-Verlag 01.01.2000
Subjects
chlorophyll
filters
fluorescence
grana
krypton
microscopy
nitrogen
temperature
thylakoids
wavelengths
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Summary:Inner structure of isolated intact chloroplasts was observed for the first time by a method of laser scanning microscopy at the temperature of liquid nitrogen at 77 K. The microscope, based on gradient index optics, has a maximum resolution of 440 nm at the wavelength of 650 nm. Chloroplasts were excited into the Q-band of chlorophyll b by a krypton laser line at 647.6 nm and fluorescence was detected using two different interference filters. The 680 nm interference filter detects the regions where photosystem (PS) 2 mainly occurs, the 730 nm interference filter detects domains with predominant location of PS1. Since PS1 occurs mainly in stroma lamellae, whereas PS2 occurs mainly in grana regions we were able to view the structure of thylakoid membrane in isolated intact chloroplast that is the closest to in vivo state.
Bibliography:http://dx.doi.org/10.1023/A:1012492919852
ISSN:0300-3604
1573-9058
DOI:10.1023/A:1012492919852