Comparison of antimicrobial activity of mycelium and spore of Ganoderma Lucidum on Prevotella Intermedia isolated from chronic periodontitis patients

• Published in: CHRISMED Journal of Health and Research Vol. 8; no. 1; pp. 35 - 39
• Main Authors: Nayak, Aarati, Dixitraj, P, Nayak, Ranganath, Bhat, Kishore
• Format: Journal Article
• Language: English
• Published: Wolters Kluwer India Pvt. Ltd 01.01.2021; Medknow Publications and Media Pvt. Ltd; Wolters Kluwer Medknow Publications
• Subjects: anaerobic bacteria; Analysis; antimicrobial agents; ganoderma lucidum; gram-negative bacteria; Periodontitis; prevotella intermedia; Anaerobic bacteria; Ganoderma lucidum; Gram-negative bacteria; antimicrobial agents; Prevotella intermedia; periodontitis
• ISSN: 2348-3334; 2348-506X
• DOI: 10.4103/cjhr.cjhr_61_20

Aim: The study aimed at comparing the antimicrobial activity of mycelium and spore of Ganoderma lucidum on Prevotella intermedia isolated from subgingival plaque of chronic periodontitis patients. Settings and Design: Written informed consent was obtained from each subject enrolled in the study. The institutional ethics committee granted ethical clearance for the study. Materials and Methods: Twenty patients diagnosed with chronic periodontitis were included in the study. Using sterile curettes, pooled subgingival plaque samples were collected from the deepest sites of periodontal pockets. The collected samples were then transported in 1 mL of reduced transport fluid. The organisms were cultured and confirmed and were used for the minimum inhibitory concentration (MIC) procedure. Statistical Analysis: Mean of the MIC value obtained was calculated. Comparison between the antimicrobial activity of mycelium and spore against P. intermedia groups with respect to mean MIC value (in mcg/ml) was done by Mann-Whitney U test. Comparison of antimicrobial activity of mycelium and spores of G. lucidum against P. intermedia expressed by the MIC value at different concentrations was done by Chi-square test. Results: The mean MIC obtained for mycelium was 5.64 mcg/ml. The mean MIC obtained for spore was 3.62 mcg/ml. Results of the comparison of antimicrobial activity implied that the antimicrobial activity of mycelium and spore of G. lucidum was comparable. Conclusions: Both mycelium and spore of G. lucidum with its bioactivity could be used as an antimicrobial, in combination with conventional therapy in periodontal disease.