Arbuscular mycorrhizal fungi build a bridge for soybeans to recruit Pseudomonas putida

• Published in: The New phytologist Vol. 246; no. 3; pp. 1276 - 1292
• Main Authors: Qiu, Wei, Kang, Jie, Ye, Zeming, Yang, Shengdie, Tu, Xiujun, Xie, Penghao, Ge, Jingping, Ping, Wenxiang, Yuan, Jun
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.05.2025
• Subjects: Acetic acid; arbuscular mycorrhizal fungi; Arbuscular mycorrhizas; Assembly; carbon; Carbon sources; cysteine; Disease resistance; Division of labor; Fungi; Funneliformis mosseae; Glomeromycota - physiology; Glomus mosseae; Glycine max; Glycine max - microbiology; Host plants; hypha secretions; Indoleacetic acid; Indoleacetic Acids - metabolism; Labor; microbial communities; microbiome; Microbiomes; Microorganisms; Molecules; Mycorrhizae - drug effects; Mycorrhizae - physiology; Plant bacterial diseases; Plant diseases; Plant growth; plant growth-promoting rhizobacteria; plant microbiome; Plant Roots - microbiology; Plants; Pseudomonas putida; Pseudomonas putida - drug effects; Pseudomonas putida - physiology; Pseudomonas putida KT2440; recruitment; Rhizosphere; root exudation; secretion; Soybeans; Symbionts; Symbiosis; Tryptophan; Tryptophan - metabolism; vesicular arbuscular mycorrhizae; arbuscular mycorrhizal fungi; root exudation; hypha secretions; recruitment; Pseudomonas putida KT2440; Funneliformis mosseae; plant microbiome
• ISSN: 0028-646X; 1469-8137; 1469-8137
• DOI: 10.1111/nph.70064

Summary The assembly of the rhizosphere microbiome determines its functionality for plant fitness. Although the interactions between arbuscular mycorrhizal fungi (AMF) and plant growth‐promoting rhizobacteria (PGPR) play important roles in plant growth and disease resistance, research on the division of labor among the members of the symbionts formed among plants, AMF, and PGPR, as well as the flow of carbon sources, is still insufficient. To address the above questions, we used soybean (Glycine max), Funneliformis mosseae, and Pseudomonas putida KT2440 as research subjects to establish rhizobiont interactions and to elucidate the signal exchange and division of labor among these components. Funneliformis mosseae can attract P. putida KT2440 by secreting cysteine as a signaling molecule and can promote the colonization of P. putida KT2440 in the soybean rhizosphere. Colonized P. putida KT2440 can stimulate the l‐tryptophan secretion of the host plant and can lead to the upregulation of genes involved in converting methyl‐indole‐3‐acetic acid (Me‐IAA) into IAA in response to l‐tryptophan stimulation. Collectively, we decipher the tripartite mechanism of rhizosphere microbial community assembly via cross‐kingdom interactions.