Micropropagation of Physalis species with economic potential

Because it has high added value, fruit production is an alternative for small products that constitute the highest income per production area (Negri et al., 2016). Asexual propagation is the main form of physalis multiplication, since this fruit has a large amount of seeds and a high germination rat...

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Published inRevista Ceres Vol. 68; no. 6; pp. 521 - 529
Main Authors Silva, Luciana Sabini Da, Villa, Fabíola, Silva, Daniel Fernandes Da, Silva, Edvan Costa Da, Ritter, Giovana, Eberling, Tatiane
Format Journal Article
LanguageEnglish
Portuguese
Published Vicosa Universidade Federal de Viçosa-UFV, Revista Ceres 01.12.2021
Universidade Federal de Viçosa
Universidade Federal De Viçosa
Subjects
AGRICULTURE, MULTIDISCIPLINARY
Asepsis
Autoclaving
Crop production
Culture media
Distilled water
Experiments
Explants
Fruits
Genetic variability
Germination
in vitro culture
Laboratories
Micropropagation
Multiplication
Orchards
Physalis sp
Plantlets
Polymer films
Polyvinyl chloride
Propagation
Seeds
Sucrose
culture media
sucrose
asepsis
Physalis sp
in vitro culture
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Summary:Because it has high added value, fruit production is an alternative for small products that constitute the highest income per production area (Negri et al., 2016). Asexual propagation is the main form of physalis multiplication, since this fruit has a large amount of seeds and a high germination rate, however it provides plantlets with high genetic variability, resulting in plants with different growth, vigor, performance and production of fruits, characteristics that are not interesting for commercial orchards (Oliveira et al., 2013). After the procedures, the seeds were washed four times in distilled water and autoclaved After the sanitization, 5 seeds were allocated by glass bottle, with a total capacity of 300 mL volum, containing 25 mL of media culture MS (Murashige & Skoog, 1962), added to 30 g L-1 of sucrose, 6 g L-1 of agar (Himedia(r)) and pH = 5,8, adjusted before autoclaving. After the autoclaving, they were sealed with plastic film of PVC, in order to avoid contaminations and kept in wooden shelves (45 x 30 cm), in a growth room, with photoperiod of 16 h light and temperature ± 24°C. In the experiments II, III and IV were used explants of pre-established plants in vitro from seeds were used.
ISSN:0034-737X
2177-3491
2177-3491
DOI:10.1590/0034-737x202168060003