The preservation of fungal cultures by lyophilization

A method for the preservation of fungal strains is presented. The cultures are grown on Sabouraud glucose agar in glass ampoules and lyophilized without further processing. By this method the macroscopical morphology of the cultures is preserved, so that these can be used immediately without reculti...

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Bibliographic Details
Published inMycoses Vol. 34; no. 3-4; p. 173
Main Authors Bunse, T, Steigleder, G K
Format Journal Article
LanguageEnglish
Published Germany 01.03.1991
Subjects
Freeze Drying
Fungi - growth & development
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Summary:A method for the preservation of fungal strains is presented. The cultures are grown on Sabouraud glucose agar in glass ampoules and lyophilized without further processing. By this method the macroscopical morphology of the cultures is preserved, so that these can be used immediately without recultivation as reference cultures. All tested mould and yeast strains remained viable over the six months duration of the experiment, whereas the dermatophyte strains could only partially be recultivated.
ISSN:0933-7407
DOI:10.1111/j.1439-0507.1991.tb00639.x