In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration

The neural crest is an embryonic cell population induced at the border of the neural plate from where it delaminates and migrates long distances across the embryo. Due to its extraordinary migratory capabilities, the neural crest has become a powerful system to study cellular and molecular aspects o...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1976; p. 135
Main Authors Barriga, Elias H, Shellard, Adam, Mayor, Roberto
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Delaunay-Voronoi triangulation
Grafts
Quantitative migration
Cell dispersion
Neural crest dissection
Xenopus laevis
In situ hybridization
Cell migration
Neural crest cells
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Summary:The neural crest is an embryonic cell population induced at the border of the neural plate from where it delaminates and migrates long distances across the embryo. Due to its extraordinary migratory capabilities, the neural crest has become a powerful system to study cellular and molecular aspects of collective and single cell migration both in vivo and in vitro. Here we provide detailed protocols used to perform quantitative analysis of molecular and cellular aspects of Xenopus laevis neural crest cell migration, both in vivo and in vitro.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9412-0_11