Virulence of entomopathogenic fungi isolated from wild mosquitoes against Aedes aegypti

• Published in: Entomological research Vol. 53; no. 4; pp. 158 - 166
• Main Authors: Aguilar‐Durán, Jesús A., Villarreal‐Treviño, Cuauhtémoc, Fernández‐Santos, Nadia A., Hamer, Gabriel L., Rodríguez‐Pérez, Mario A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 01.04.2023
• Subjects: Aedes aegypti; Aspergillus tamarii; Beauveria bassiana; Conidia; Culicidae; dengue; Entomopathogenic fungi; Fecundity; Fungi; humans; insects; Metarhizium anisopliae; Mexico; Mosquitoes; Trichoderma; Vectors; Virulence; Mexico
• ISSN: 1738-2297; 1748-5967
• DOI: 10.1111/1748-5967.12640

The entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana are highly virulent control tools for insect pests and have been under evaluation for the control of globally important mosquito vectors such as Aedes aegypti . Here, we identified and isolated other virulent entomopathogenic fungi against Ae. aegypti . We collected 7 species of mosquitoes by human landing catch in 5 municipalities in Central and Northern Mexico and isolated 28 species of fungi. We harvested fungal conidia from six and assessed virulence against Ae. aegypti females. We observed variation in virulence of fungi in Ae. aegypti with the most virulent being Aspergillus tamarii , with a LT 50 of 6.4 (±0.65) days and the least virulent was Trichoderma euskadiense with a LT 50 of 16.3 (±1.5) days. Additional assays evaluated the impact of the fungi on Ae. aegypti fecundity and fertility and A. tamarii had the highest for both, resulting in 60% and 37% decrease, respectively. These results provide support for the potential utility of A. tamarii as an entomopathogenic control tool for the dengue vector, Ae. aegypti , pending further evaluations of environmental and nontarget safety.