Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge

This study presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a...

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Published inBiointerphases Vol. 11; no. 2; p. 011009
Main Authors Romero-Mangado, Jaione, Nordlund, Dennis, Soberon, Felipe, Deane, Graham, Maughan, Kevin, Sainio, Sami, Singh, Gurusharan, Daniels, Stephen, Saunders, Ian T, Loftus, David, Meyyappan, M, Koehne, Jessica, Gandhiraman, Ram P
Format Journal Article
LanguageEnglish
Published United States AIP 01.03.2016
Subjects
Aerosols
bacteria
BASIC BIOLOGICAL SCIENCES
carbon
Electricity
Escherichia coli - chemistry
Escherichia coli - cytology
Escherichia coli - physiology
Fourier transform infrared spectroscopy
Microbial Viability
Microscopy, Electron
Oxidation-Reduction
Spectroscopy, Fourier Transform Infrared
Surface Properties
X-ray absorption near edge structure
X-Ray Absorption Spectroscopy
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Summary:This study presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reduction in amine functionalities and phosphate groups. Near edge x-ray absorption fine structure (NEXAFS) measurements confirm the presence of additional oxide bonds upon DBD treatment, suggesting oxidation of the outer layer of the cell wall. Electron microscopy images show that the bacteria undergo physical distortion to varying degrees, resulting in deformation of the bacterial structure. The electromagnetic field around the DBD coil causes severe damage to the cell structure, possibly resulting in leakage of vital cellular materials. The oxidation and chemical modification of the bacterial components are evident from the Fourier transform infrared spectroscopy and NEXAFS results. The bacterial reculture experiments confirm inactivation of airborne E. coli upon treating with DBD.
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USDOE Office of Science (SC), Basic Energy Sciences (BES)
AC02-76SF00515
ISSN:1934-8630
1559-4106
DOI:10.1116/1.4941367