Fresnel lens optical fiber tweezers to evaluate the vitality of single algae cells

Dunaliella salina algae are trapped and studied using dual-fiber optical tweezers based on nano-imprinted Fresnel lenses. Different forms of cyclic motion of living algae inside the optical trap are observed and analyzed. A characteristic periodic motion in the 0-35 Hz frequency region reflects the...

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Bibliographic Details
Published inOptics letters Vol. 47; no. 1; p. 170
Main Authors Asadollahbaik, Asa, Kumar, Aashutosh, Heymann, Michael, Giessen, Harald, Fick, Jochen
Format Journal Article
LanguageEnglish
Published United States 01.01.2022
Subjects
Motion
Optical Fibers
Optical Tweezers
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Summary:Dunaliella salina algae are trapped and studied using dual-fiber optical tweezers based on nano-imprinted Fresnel lenses. Different forms of cyclic motion of living algae inside the optical trap are observed and analyzed. A characteristic periodic motion in the 0-35 Hz frequency region reflects the algal flagella activity and is used to estimate the algal vitality, by photomovement. The trap stiffness and optical forces are measured for the case of a dead algal cell. It is shown that the dual-fiber optical tweezers can be used to study the vitality (or viability) property of single cells, a property that is essential and can be scaled up to other applications, such as sperm analysis for fertility tests.
ISSN:1539-4794
DOI:10.1364/OL.447683