DNA binding and photocleavage properties of two mixed-ligand oxovanadium complexes

• Published in: Transition metal chemistry (Weinheim) Vol. 35; no. 7; pp. 859 - 864
• Main Authors: Du, Yi-fan, Lu, Jia-Zheng, Guo, Hai-wei, Jiang, Jing, Chao, Ping, Chen, Fei, Pan, Jie-Ming
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2010; Springer Nature B.V
• Subjects: Catalysis; Chemistry; Chemistry and Materials Science; Inorganic Chemistry; Organometallic Chemistry; Physical Chemistry; Viscosity Measurement; Thermal Denaturation; Absorption Titration; Thiosemicarbazone; Bipy
• ISSN: 0340-4285; 1572-901X
• DOI: 10.1007/s11243-010-9404-8

An oxovanadium complex [VO(satsc)(bipy)] ( 1) (satsc = salicylaldehyde thiosemicarbazone, bipy = 2,2′-bipyridine) and its dibromo derivative [VO(3,5-dibrsatsc)(bipy)] ( 2) (3,5-dibrsatsc = 3,5-dibromosalicylaldehyde thiosemicarbazone) have been synthesized and characterized by elemental analysis, IR, ES-MS and 1 H NMR. The interaction of these two complexes with calf-thymus DNA (CT-DNA) was studied using UV/Vis, fluorescence spectroscopic titration, viscosity measurements and thermal denaturation. The results suggest that complexes 1 and 2 interact with CT-DNA by intercalative modes. The DNA-binding affinity of complex 1 is larger than that of complex 2 . In addition, their photocleavage reactions with pBR322 supercoiled plasmid DNA were investigated by gel electrophoresis experiments. Both complexes exhibit significant DNA cleavage activity, and complex 1 showed greater cleaving efficiency than complex 2.