A case of Bordetella trematum and Kerstersia gyiorum infections in a patient with congestive dermatitis

• Published in: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Vol. 27; no. 5; pp. 740 - 746
• Main Authors: Kitagawa, Daisuke, Kurimoto, Toru, Oyama, Shinichiro, Suzuki, Soma, Masuo, Kazue, Suzuki, Yuki, Nakano, Ryuichi, Sato, Masatoshi, Yano, Hisakazu, Maeda, Koichi, Nomi, Kyoko, Nakamura, Fumihiko
• Format: Journal Article
• Language: English
• Published: Netherlands 01.05.2021
• Subjects: 16S ribosomal RNA gene sequencing; Bordetella trematum; Kerstersia gyiorum; Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry
• ISSN: 1341-321X; 1437-7780
• DOI: 10.1016/j.jiac.2020.12.008

Bordetella trematum and Kerstersia gyiorum are rare gram-negative bacilli that are not frequently detected in human infections. In this report, we describe a case of a 48-year-old man who presented to our hospital with an infected wound on his leg. Discharges from the cracks of the granulation were collected and evaluated in our microbiology laboratory. Gram staining of the specimen showed polymorphonuclear leukocytes and abundant gram-negative bacilli. Three types of colonies were isolated on blood agar and were identified as B. trematum and Alcaligenes faecalis using VITEK MS. Moreover, K. gyiorum and B. trematum were identified and confirmed via 16S ribosomal RNA (rRNA) gene sequencing. The patient successfully recovered following application of meropenem antibacterial therapy and surgical debridement. This is the first reported case of complex wound infection caused by both B. trematum and K. gyiorum. Identification of B. trematum has recently been made possible by routine bacterial identification using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). However, K. gyiorum isolation is still rare, and species identification requires 16S rRNA sequencing. Thus, this case highlighted the importance of using multiple methods, such as MALDI-TOF MS and 16S rRNA gene sequencing, for identification of rarely isolated species from clinical specimens.