Kinetic evaluation and study of gold-based nanoparticles and multi-walled carbon nanotubes as an alkaline phosphatase inhibitor in serum and pure form

• Published in: Carbon Letters Vol. 33; no. 4; pp. 1217 - 1229
• Main Authors: Mohammed, Samar Naser, Al-Rawi, Khalid F., Mohammed, Ahmed Mishaal
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.06.2023; 한국탄소학회; Springer Nature B.V
• Subjects: Alkaline phosphatase; Aqueous solutions; Atomic force microscopy; Automation; Biocompatibility; Biomarkers; Carbon; Characterization and Evaluation of Materials; Chemical synthesis; Chemistry and Materials Science; Disease; Electron microscopy; Energy dispersive X ray analysis; Enzymatic activity; Enzyme activity; Enzymes; Fourier transforms; Gold; Green chemistry; Infrared spectroscopy; Kinases; Materials Engineering; Materials Science; Microscopy; Multi wall carbon nanotubes; Nanoparticles; Nanotechnology; Nanotubes; Original Article; Orthophosphate; Oxidation; Phosphatase; Phosphate esters; Scanning electron microscopy; Substrates; Sulfuric acid; Transmission electron microscopy; X ray analysis; MWCNTs; Green chemistry; Walnut shell; AuNPs; ALP enzyme; MWCNTs · AuNPs · Green chemistry · Walnut shell · ALP enzyme
• ISSN: 1976-4251; 2233-4998
• DOI: 10.1007/s42823-023-00509-z

In this study, gold nanoparticles (AuNPs) were synthesised using green chemistry to decorate multi-walled carbon nanotubes (MWCNTs) made from walnut shells transmission electron microscopy, field-emission scanning electron microscopy (FESEM), atomic force microscopy and fourier transforms infrared spectroscopy were used to diagnose MWCNTs and AuNPs. MWCNT-COOAu, MWCNT-COO and MWCNT-Au were diagnosed by Raman, energy dispersive X-ray analysis and FESEM. The effect of AuNPs, MWCNT-COO, MWCNT-COOAu and MWCNT-Au on pure and serum alkaline phosphatase (ALP) enzyme activity was studied in vitro using the enzyme-substrate 4-nitrophenyl disodium orthophosphate. For pure enzymes, V max slightly increased as the concentration of MWCNT-Au, MWCNT-COOAu and MWCNTCOO increased, whereas the V max values decreased as the concentration of AuNPs increased. The inhibition type for all NPs varied. For serum ALP enzyme, the V max values for Au-based NPs decreased as the concentration of NPs increased. The V max values exceeded the standard value at the concentrations of 25, 50 and 75 ppm for MWCNT-Au and MWCNT-COOAu, whereas the V max values increased over the standard value for all concentrations of AuNPs.