Separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography

Methods were developed for the extraction and separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography (RP-ICP). Nucleotides (ATP, ADP, and AMP) were separated using a mobile phase of 15 mM TBAHS, 15 mM KH2PO4, and 7% acetonitrile. Inositol hexakisphosphate was sep...

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Bibliographic Details
Published inCommunications in soil science and plant analysis Vol. 34; no. 9-10; pp. 1393 - 1406
Main Authors Leytem, A.B, Mikkelsen, R.L
Format Journal Article
LanguageEnglish
Published Taylor & Francis Group 01.01.2003
Subjects
adenosine diphosphate
adenosine monophosphate
adenosine triphosphate
extraction
inositol phosphates
ion pair chromatography
organophosphorus compounds
soil analysis
soil organic matter
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Summary:Methods were developed for the extraction and separation of soil organic phosphorus compounds using reverse-phase ion-pair chromatography (RP-ICP). Nucleotides (ATP, ADP, and AMP) were separated using a mobile phase of 15 mM TBAHS, 15 mM KH2PO4, and 7% acetonitrile. Inositol hexakisphosphate was separated using a mobile phase composition of 0.05 M formic acid:methanol (49:51 v/v) and 1.5 mL/100 mL of TBAOH. Extraction procedures were developed for the nucleotides which would be compatible with the RP-ICP system developed for their separation.
ISSN:0010-3624
1532-2416
DOI:10.1081/CSS-120020452