Bilateral adrenal enucleation-induced changes in adenohypophyseal pro-opiomelanocortin (POMC)-related peptides synthesis and secretion : A comparative study with adrenalectomized rats

• Published in: Journal of endocrinological investigation Vol. 20; no. 4; pp. 172 - 182
• Main Authors: PERONE, M. J, CHISARI, A. N, GOMEZ DUMM, C. L, SPINEDI, E, ESTIVARIZ, F. E
• Format: Journal Article
• Language: English
• Published: Milano Kurtis 01.04.1997
• Subjects: Adrenal Glands - pathology; Adrenal Glands - physiology; Adrenal Glands - surgery; Adrenalectomy; Adrenocorticotropic Hormone - blood; Animals; beta-Endorphin - blood; Biological and medical sciences; Corticosterone - blood; Female; Functional investigation of endocrine glands and genital system; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Peptide Biosynthesis; Peptides - secretion; Pituitary Gland, Anterior - metabolism; Pituitary Gland, Anterior - physiology; Pituitary Gland, Anterior - secretion; Pro-Opiomelanocortin - biosynthesis; Pro-Opiomelanocortin - secretion; Rats; Rats, Sprague-Dawley; Regeneration; Time Factors; Animal model; Rat; Adrenal glands; Steroid hormone; Rodentia; Bilateral; Biosynthesis; Prohormone; Glucocorticoid; Endocrine secretion; Enucleation; Vertebrata; Mammalia; Surgery; Animal; Adrenal hormone; Proopiocortin; Biological effect; Comparative study
• ISSN: 0391-4097; 1720-8386
• DOI: 10.1007/BF03346899

The aim of the present study was to elucidate the modulatory effect of transient changes in endogenous glucocorticoids, occurring after bilateral adrenal enucleation (ENUC), on anterior pituitary (AP) proopiomelanocortin (POMC)-derived peptides synthesis and output in rats. For this purpose, adult female rats were either bilaterally ENUC, adrenalectomized (ADX), or sham-operated (SHAM) and killed by decapitation 2, 7, 14, and 21 days after surgery. Trunk blood was collected for measurements of ACTH, beta-endorphin (beta-END) and corticosterone (B) concentrations; APs were quickly dissected for the determination of ACTH, beta-endorphin (beta-END)-like (beta-END-LI) and gamma 3-MSH contents and adrenal glands were removed and submitted to histological study. The results indicate that ENUC and ADX increased AP POMC-related peptides synthesis and release in association with changes in the AP processing of peptides belonging to the N-terminal (gamma 3-MSH), mid (ACTH) and C-terminal (beta-LPH/ENDs) portions of POMC. While ADX abolished plasma B levels, ENUC induced a transient (day 2) decrease in plasma B concentrations which returned to SHAM levels at 7 days after surgery. These data tallied with the histological observations carried out, indicating a time-dependent regenerative process of the adrenal which was completed by three weeks after ENUC. There was a different pattern in plasma ACTH and beta-END levels between ENUC and ADX; maximal plasma peptide levels were found 7-14 days after ENUC, then falling down to SHAM values at 21 days post ENUC. Conversely, there was a constant increment in plasma peptide levels up to 21 days after ADX. At 2 days after both ENUC and ADX all peptides measured in the AP were lower than SHAM values, thus reflecting a rapid corticotrope secretion. Thereafter, 7 or more days after surgery, AP peptide content in ADX rats increased, in a time-related fashion, up to 21 days after surgery. Only beta-END-LI showed a similar AP content to that of the SHAM group, thereafter indicating a preferential cleavage of POMC to beta-END long after ADX (21 days). ENUC rats showed increased AP POMC peptides content throughout the whole time, and it was significantly different from SHAM and ADX values 14 days post-surgery. Interestingly, we found an increment in AP gamma 3-MSH, a peptide which is preferentially synthesized in the intermediate lobe of the rat pituitary, in both ENUC and ADX situations. Our results further indicate that: 1) glucocorticoids, from regenerating adrenal origin, induce a fast negative feedback mechanism on AP secretion, and 2) there might be a delayed inhibitory action of newly synthesized corticosteroids on higher levels of the central nervous system. The lack of glucocorticoids (ADX) clearly corroborates a persistent enhancement of AP POMC-related peptides synthesis and secretion. The differences in AP processing of POMC between ENUC and ADX might be due to qualitative/quantitative changes in hypothalamic ACTH secretagogues output.