In vitro effects of fermented papaya ( Carica papaya , L.) on platelets obtained from patients with type 2 diabetes

• Published in: Nutrition, metabolism, and cardiovascular diseases Vol. 25; no. 2; pp. 224 - 229
• Main Authors: Raffaelli, F, Nanetti, L, Montecchiani, G, Borroni, F, Salvolini, E, Faloia, E, Ferretti, G, Mazzanti, L, Vignini, A
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2015
• Subjects: Antioxidants - pharmacology; Blood Platelets - metabolism; Cardiovascular; Carica; Case-Control Studies; Diabetes; Diabetes Mellitus, Type 2 - blood; Female; Fermentation; Food Handling; Healthy Volunteers; Humans; Insulin - blood; Insulin - metabolism; Insulin Resistance; Insulin Secretion; Male; Membrane fluidity; Middle Aged; Na+/K+-ATPase; Oxidative Stress; Plant Preparations - pharmacology; Platelets; Sodium-Potassium-Exchanging ATPase - metabolism; Superoxide Dismutase - metabolism; Oxidative stress; Membrane fluidity; Diabetes; Na +/K +-ATPase; Platelets; Na+/K+-ATPase; Na(+)/K(+)-ATPase
• ISSN: 0939-4753; 1590-3729
• DOI: 10.1016/j.numecd.2014.10.013

Abstract Background and aim Oxidative stress is associated with insulin resistance pathogenesis, insulin secretion deficiency, and complication onset. Fermented papaya preparation (FPP), a dietary supplement obtained by fermentation of the papaya fruit, may be used as an antioxidant in the prevention of diabetic complications. Methods and results Platelets from 30 patients with type 2 diabetes mellitus (DM 2) and 15 healthy subjects were analyzed to evaluate the in vitro effects of FPP incubation. Na+ /K+ -adenosine triphosphatase (ATPase) activity, membrane fluidity, total antioxidant capacity (TAC), superoxide dismutase (SOD) activity, and conjugated diene levels were determined. In vitro FPP incubation improved platelet function, by enhancing Na+ /K+ -ATPase activity and membrane fluidity, and ameliorated the antioxidant system functionality, through an increase in TAC and SOD activity and a parallel decrease in conjugated diene levels in patients with DM 2. Conclusion Our data suggest that the incubation with FPP may have a protective effect on platelets from patients with DM 2, by preventing the progression of oxidative damage associated with diabetes and its complications.