Nitrogen Form Alters Hormonal Balance in Salt-treated Tomato (Solanum lycopersicum L.)

• Published in: Journal of plant growth regulation Vol. 30; no. 2; pp. 144 - 157
• Main Authors: Ghanem, Michel Edmond, Martínez-Andújar, Cristina, Albacete, Alfonso, Pospíšilová, Hana, Dodd, Ian C., Pérez-Alfocea, Francisco, Lutts, Stanley
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.06.2011; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Life Sciences; Lycopersicon esculentum; Plant Anatomy/Development; Plant Physiology; Plant Sciences; Solanum; Ammonium; L.; Salt stress; Indole-3-acetic acid; Zeatin riboside; Sodium chloride; Tomato; Cytokinin; Plant hormones; Zeatin; Nitrate; Nitrogen metabolism; Abscisic acid
• ISSN: 0721-7595; 1435-8107
• DOI: 10.1007/s00344-010-9178-4

Mixed nitrate/ammonium fertilization can partially alleviate the negative effects of salinity on growth of some plant species compared to all-nitrate or all-ammonium fertilization. To gain insights about the mechanisms involved, tomato ( Solanum lycopersicum L. cv Moneymaker) plants were grown hydroponically for 3 weeks with two NO 3 − /NH 4 + fertilization regimes (6/0.5 and 5/1.5; N total  = 6.5 mM) in the absence (control) or presence of salt stress (100 mM NaCl). Ammonium enrichment had no effect on growth and other parameters under control conditions. Under salinity, however, ammonium enrichment improved shoot and root biomass by 20% and maintained leaf PSII efficiency close to control levels. These changes were related to higher leaf K + , NO 3 − , and NH 4 + concentrations and activities of the N-assimilatory enzymes glutamate synthase (GOGAT) and glutamine synthase (GS) in the leaves. Ammonium enrichment also attenuated the salt-induced increase in leaf abscisic acid (ABA) concentration and decrease in leaf concentrations of indole 3-acetic acid (IAA) and the cytokinins trans -zeatin ( t Z) and trans -zeatin riboside ( t ZR). Enhanced cytokinin status was probably due to maintenance of root-to-shoot cytokinin transport and decreased leaf induction of the cytokinin-degrading enzyme cytokinin oxidase/dehydrogenase (CKX) under ammonium-enriched conditions. It is concluded that nitrogen form modifies salinity-induced physiological responses and that these modifications are associated with changes in plant hormone status.