Relationship between transmembrane ion movements, production of reactive oxygen species and the hypersensitive response during the challenge of tobacco suspension cells by zoospores of Phytophthora nicotianae

• Published in: Physiological and molecular plant pathology Vol. 58; no. 5; pp. 189 - 198
• Main Authors: Able, A.J., Guest, D.I., Sutherland, M.W.
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 01.05.2001; Elsevier
• Subjects: Biological and medical sciences; calcium; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; hydrogen peroxide; hypersensitive response; Nicotiana tabacum; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; Phytophthora nicotianae; plant–pathogen interactions; potassium; ROS; superoxide; XTT; plant–pathogen interactions; calcium; potassium; hydrogen peroxide; ROS; Phytophthora nicotianae; pH; XTT; HR; Nicotiana tabacum; superoxide; AOS (active oxygen species); Cell culture; Plant pathogen; Potassium ion; Phycomycetes; Cell biology; Stimulant plant; Biochemistry; Spores; Fungi; Suspension culture; Calcium ion; Membrane transport; Dicotyledones; Angiospermae; Solanaceae; Thallophyta; Cell oxidation; Pathophysiology; Hypersensitivity; Ion transport; Host agent relation; Experimental study; Hydrogen Peroxides; In vitro; Physiologic race; Fungus resistance; Hyperoxides; Spermatophyta; Experimental plant
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1006/pmpp.2001.0328

We have examined the responses of suspension cultured tobacco cells after inoculation with zoospores from compatible or incompatible races of the Oomycete pathogen Phytophthora nicotianae. The incompatible interaction, characterized by the production of reactive oxygen species (ROS) (specifically superoxide (<$>{\rm HO^{\,\cdot}_{2}/O_{2}^{\minus}}<$>) and hydrogen peroxide (H2O2)), and by a hypersensitive response (HR), was not dependent upon the presence of exogenous calcium. However, perturbation of calcium ion movements by EGTA or LaCl3suppressed both ROS production and the extent of cell death. The Ca2+-specific ionophore, A23187, slightly enhanced ROS production during the incompatible interaction but had no effect on the responses of susceptible or unchallenged cells. These results confirm that both ROS production and the HR are potentiated by movement of endogenous calcium across the plasmalemma. While there was no evidence of a transmembrane K+/H+exchange immediately following incompatible zoospore challenge, a later potassium efflux from cells coincided with the onset of the HR. Unexpectedly, medium pH drifted downwards during all host cell responses suggesting that alkaline peroxidases are not the major source of ROS generation during an incompatible tobacco cell/zoospore interaction.