Growth Properties and Cholesterol Removal Ability of Electroporated Lactobacillus acidophilus BT 1088

• Published in: Journal of microbiology and biotechnology Vol. 22; no. 7; pp. 981 - 989
• Main Authors: Lye, H.S., Universiti Sains Malaysia, Penang, Malaysia, Khoo, B.Y., Universiti Sains Malaysia, Penang, Malaysia, Karim, A.A., Universiti Sains Malaysia, Penang, Malaysia, Rusul, G., Universiti Sains Malaysia, Penang, Malaysia, Liong, M.T., Universiti Sains Malaysia, Penang, Malaysia
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.07.2012; 한국미생물·생명공학회
• Subjects: Bacterial Adhesion; Biological and medical sciences; Biotechnology; Cell Line; Cell Membrane - chemistry; Cell Membrane - physiology; CHOLESTEROL; Cholesterol - metabolism; COLESTEROL; ELECTROPORACION; ELECTROPORATION; ENFOUISSEMENT; ENTERRADO; Epithelial Cells - microbiology; Fundamental and applied biological sciences. Psychology; Humans; INCORPORATION; lactobacilli; Lactobacillus acidophilus - growth & development; Lactobacillus acidophilus - metabolism; Lactobacillus acidophilus - physiology; MEMBRANA; MEMBRANE; MEMBRANES; passage; 생물학; Lactic acid bacteria; Incorporation; passage; Growth; Bacteria; Lactobacillaceae; Electroporation; Membrane; lactobacilli; Lactobacillus acidophilus; Cholesterol
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1201.12073

This study aimed to evaluate the effects of electroporation on the cell growth, cholesterol removal, and adherence abilities of L. acidophilus BT 1088 and their subsequent passages. The growth of electroporated parent cells increased (P less than 0.05) by 4.49-21.25% compared with that of the control. This may be attributed to the alteration of cellular membrane. However, growth of first, second, and third passages of treated cells was comparable with that of the control, which may be attributed to the resealing of transient pores on the cellular membrane. Electroporation also increased (P less than 0.05) assimilation of cholesterol by treated parent cells (greater than 185.40%) and first passage (greater than 21.72%) compared with that of the control. Meanwhile, incorporation of cholesterol into the cellular membrane was also increased (P less than 0.05) in the treated parent cells (greater than 108.33%) and first passage (greater than 26.67%), accompanied by increased ratio of cholesterol:phospholipids (C:P) in these passages. Such increased ratio was also supported by increased enrichment of cholesterol in the hydrophilic heads, hydrophobic tails, and the interface regions of the membrane phospholipids of both parent and first passage cells compared with that of the control. However, such traits were not inherited by the subsequent second and third passages. Parent cells also showed decreased intestinal adherence ability (P less than 0.05; decreased by 1.45%) compared with that of the control, without inheritance by subsequent passages of treated cells. Our data suggest that electoporation could be a potential physical treatment to enhance the cholesterol removal ability of lactobacilli that was inherited by the first passage of treated cells without affecting their intestinal adherence ability.