Production of optically pure L-alanine in batch and continuous stirred tank reactors using immobilized Pseudomonas sp

A process to obtain optically pure L-alanine has been developed using batch and continuous stirred tank reactors with a new L-aminoacylase-producing bacterium Pseudomonas sp. BA2 immobilized in calcium alginate beads coated with glutaraldehyde. The maximum production of L-alanine in a continuous sti...

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Bibliographic Details
Published inBiotechnology letters Vol. 23; no. 11; pp. 887 - 891
Main Authors Hernandez, H, Asanza, M.L, Alcaraz, I, Hidalgo, A.M, Maximo, M.F, Bastida, J
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.06.2001
Springer Nature B.V
Subjects
alanine
Bacteria
Batch processing
Biological and medical sciences
Biotechnology
calcium alginate
Chemical reactors
Fundamental and applied biological sciences. Psychology
glutaraldehyde
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Pseudomonas
Reactors
Pseudomonadales
Alanine
Bioreactor
Batchwise
Production
Bacteria
Pseudomonadaceae
Optical purity
Pseudomonas
Continuous stirred tank reactor
Entrapped microorganism
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Summary:A process to obtain optically pure L-alanine has been developed using batch and continuous stirred tank reactors with a new L-aminoacylase-producing bacterium Pseudomonas sp. BA2 immobilized in calcium alginate beads coated with glutaraldehyde. The maximum production of L-alanine in a continuous stirred tank reactor was 11.26 g after 2 days of operation which is higher than that previously reported.
Bibliography:http://www.kluweronline.com/issn/0141-5492/contents
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1010524110121