Brewing of sake containing glutathione

We discovered that a mutant defective in vacuolar functions (slp 1-1 mutant) leaked glutathione, but strain KL-197-12 B (slp 1-1 mutant) had weak fermentation. Therefore, hybrids were bred by repeated crossings between a temporal parent KL-197-12 B and a repetitive parent Kyou-kai No. 7 (K-7). Strai...

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Published inNippon Nōgeikagaku Kaishi Vol. 65; no. 7; pp. 1089 - 1095
Main Authors SAWANO, Yasuji, FUJII, Fumiko, KANDA, Akihiro, HAMACHI, Masaaki
Format Journal Article
LanguageJapanese
Published Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 1991
Nippon Nōgei Kagakukai
Subjects
alcoholic beverages
bebidas alcoholicas
boisson alcoolisee
brassage
brewing
malteado
peptide
peptides
peptidos
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Summary:We discovered that a mutant defective in vacuolar functions (slp 1-1 mutant) leaked glutathione, but strain KL-197-12 B (slp 1-1 mutant) had weak fermentation. Therefore, hybrids were bred by repeated crossings between a temporal parent KL-197-12 B and a repetitive parent Kyou-kai No. 7 (K-7). Strain KL-197-12 B (α) was crossed with haploids of strain K-7 to yield first generation-haploids (1 HY). The haploids selected from 1 HY were back-crossed with haploids of K-7 to yield second generation-hybrids (2 HY), and the same process was continued until the strains had restored fermentation. Several strains were comparable to K-7 on fermentation of sake mash at a laboratory scale. Strain (No. 133) was most suitable in glutathione-leakage and fermentation.
Bibliography:Q
Q02
ISSN:0002-1407
1883-6844
DOI:10.1271/nogeikagaku1924.65.1089