RotoStep: A Chromosome Dynamics Simulator Reveals Mechanisms of Loop Extrusion

• Published in: Cold Spring Harbor Symposia on Quantitative Biology
• Main Authors: Lawrimore, Josh, Friedman, Brandon, Doshi, Ayush, Bloom, Kerry
• Format: Journal Article
• Language: English
• Published: United States 01.01.2017
• ISSN: 1943-4456
• DOI: 10.1101/sqb.2017.82.033696

ChromoShake is a three-dimensional simulator designed to explore the range of configurational states a chromosome can adopt based on thermodynamic fluctuations of the polymer chain. Here, we refine ChromoShake to generate dynamic simulations of a DNA-based motor protein such as condensin walking along the chromatin substrate. We model walking as a rotation of DNA-binding heat-repeat proteins around one another. The simulation is applied to several configurations of DNA to reveal the consequences of mechanical stepping on taut chromatin under tension versus loop extrusion on single-tethered, floppy chromatin substrates. These simulations provide testable hypotheses for condensin and other DNA-based motors functioning along interphase chromosomes. Our model reveals a novel mechanism for condensin enrichment in the pericentromeric region of mitotic chromosomes. Increased condensin dwell time at centromeres results in a high density of pericentric loops that in turn provide substrate for additional condensin.