bcl2, bax, and nestin in the brains of patients with neurodegeneration and those of normal aging

• Published in: Journal of molecular neuroscience Vol. 27; no. 2; pp. 167 - 174
• Main Authors: Lu, Gang, Kwong, W H, Li, Qi, Wang, Xicai, Feng, Zhongtang, Yew, David T
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.01.2005
• Subjects: Aged; Aged, 80 and over; Aging; Aging - metabolism; bcl-2-Associated X Protein - metabolism; Brain - cytology; Brain - metabolism; Humans; Immunohistochemistry; Intermediate Filament Proteins - metabolism; Nerve Tissue Proteins - metabolism; Nestin; Neurodegenerative Diseases - metabolism; Proto-Oncogene Proteins c-bcl-2 - metabolism
• ISSN: 0895-8696; 0895-8696; 1559-1166
• DOI: 10.1385/JMN:27:2:167

This study was conducted by employing specimens from the frontal cortices of Alzheimer, multiple-infarct dementia patients, and those of normal aging (age matched to patients). The objective was to evaluate and compare the bcl2, bax, and nestin patterns in these three groups. Using immunocytochemistry, it was observed that bcl2 and bax active sites were colocalized in 45% of cells in Alzheimer, 52% of cells in multiple infarct, and 30% of cells in normal aging. bcl2 and bax could also be separately located in cells of all three groups. bax cells were most prominent in number in Alzheimer patients and least prominent in normal aging. nestin was found in all three groups but was most prominent in the multiple-infarct patients. Both astrocytes and neurons demonstrated positive nestin sites. The difference in pattern between groups will lead to further understanding of cellular changes in neurodegenerative patients and those of normal aging.