Sulforaphane Bioavailability and Effects on Blood Pressure in Women with Pregnancy Hypertension

• Published in: Reproductive sciences (Thousand Oaks, Calif.) Vol. 28; no. 5; pp. 1489 - 1497
• Main Authors: Langston-Cox, A. G., Anderson, D., Creek, D. J., Palmer, K. R., Marshall, S. A., Wallace, E. M.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.05.2021
• Subjects: Adult; Biological Availability; Blood Pressure - drug effects; Cross-Over Studies; Embryology; Female; Humans; Hypertension, Pregnancy-Induced - metabolism; Isothiocyanates - administration & dosage; Isothiocyanates - pharmacokinetics; Maternal Fetal Medicine/Biology: Original Article; Medicine; Medicine & Public Health; Obstetrics/Perinatology/Midwifery; Pre-Eclampsia - metabolism; Pregnancy; Reproductive Medicine; Sulfoxides - administration & dosage; Sulfoxides - pharmacokinetics; Young Adult; Broccoli; Bioavailability; Antioxidant; Preeclampsia; Sulforaphane
• ISSN: 1933-7191; 1933-7205
• DOI: 10.1007/s43032-020-00439-5

Sulforaphane, an isothiocyanate found in cruciferous vegetables such as broccoli, shows promise as an adjuvant therapy for preeclampsia. To inform future clinical trials, we set out to determine the bioavailability of sulforaphane in non-pregnant and preeclamptic women. In six healthy female volunteers, we performed a crossover trial to compare the bioavailability of sulforaphane and metabolites afforded by an activated and non-activated broccoli extract preparation. We then undertook a dose escalation study of the activated broccoli extract in 12 women with pregnancy hypertension. In non-pregnant women, an equivalent dose of activated broccoli extract gave higher levels of sulforaphane and metabolites than a non-activated extract ( p  < 0.0001) and greater area under the curve (AUC) (3559 nM vs. 2172 nM, p  = 0.03). Compared to non-pregnant women, in women with preeclampsia, the same dose of activated extract gave lower levels of total metabolites ( p  < 0.000) and AUC (3559 nM vs. 1653 nM, p  = 0.007). Doubling the dose of the activated extract in women with preeclampsia doubled levels of sulforaphane and metabolites ( p  = 0.02) and AUC (1653 nM vs. 3333 nM, p  = 0.02). In women with preeclampsia, activated broccoli extract was associated with modest decreases in diastolic blood pressure ( p  = 0.05) and circulating levels of sFlt-1 ( p  = 0.0002). A myrosinase-activated sulforaphane formulation affords better sulforaphane bioavailability than a non-activated formulation. Higher doses of sulforaphane are required to achieve likely effective doses in pregnant women than in non-pregnant women. Sulforaphane may improve endothelial function and blood pressure in women with pregnancy hypertension.