Monitoring base excision repair proteins on damaged DNA using human cell extracts

BER (base excision repair) is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). We have developed an assay, using formaldehyde cross-linking during repair in human cell extracts, to observe BER proteins involved in the repair of damaged DNA....

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Bibliographic Details
Published inBiochemical Society transactions Vol. 32; no. Pt 6; p. 962
Main Authors Parsons, J L, Dianov, G L
Format Journal Article
LanguageEnglish
Published England 01.12.2004
Subjects
DNA Damage
DNA Repair - genetics
DNA, Neoplasm - genetics
Formaldehyde
HeLa Cells
Humans
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Summary:BER (base excision repair) is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). We have developed an assay, using formaldehyde cross-linking during repair in human cell extracts, to observe BER proteins involved in the repair of damaged DNA. This approach allows visualization of repair proteins on damaged DNA during BER in human cell extracts and provides a detailed view of the molecular events leading to repair.
ISSN:0300-5127
DOI:10.1042/BST0320962