Mitochondrial calcium handling in normotensive and spontaneously hypertensive rats: correlation with systolic blood pressure levels

• Published in: Mitochondrion Vol. 20; pp. 75 - 81
• Main Authors: Pardo, Alejandro Ciocci, Rinaldi, Gustavo J, Mosca, Susana M
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.2015
• Subjects: Animals; Blood Pressure; Calcium - metabolism; Membrane Potential, Mitochondrial - drug effects; Mitochondria - drug effects; Mitochondria - metabolism; Rats, Inbred SHR; Rats, Inbred WKY; Ca(2+) response; Ca(2+)-retention capacity; WKY; ΔΨm; SHR; mPTP
• ISSN: 1567-7249; 1872-8278
• DOI: 10.1016/j.mito.2014.12.003

The aim was to study the mitochondrial Ca(2+) handling of mitochondria isolated from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) hearts and to establish a possible correlation with systolic blood pressure (SBP). Mitochondrial swelling after Ca(2+) addition, Ca(2+)-retention capacity (CRC) by calcium green method, and membrane potential (ΔΨm) were assessed. SBP was 124±1 (WKY) and 235±6mmHg (SHR). CRC, Ca(2+) response and ΔΨm were lower in SHR than WKY mitochondria. The conclusion is: the more depolarized state of SHR than WKY mitochondria results in an abnormal Ca(2+) handling and this event is closely associated with the SBP.