CD5+ B lymphocytes secrete IL-10 rather than TGF-β1 which control the immune response in autoimmune haemolytic anaemia/Evans syndrome

• Published in: Autoimmunity (Chur, Switzerland) Vol. 52; no. 1; pp. 12 - 20
• Main Authors: Zhao, Manjun, Duan, Ningning, Wang, Yi, Zhu, Hongli, Liu, Hong, Wang, Huaquan, Xing, Limin, Shao, Zonghong
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.01.2019; Taylor & Francis Group
• Subjects: Adolescent; Adult; Aged; Anemia, Hemolytic, Autoimmune - blood; Anemia, Hemolytic, Autoimmune - diagnosis; Anemia, Hemolytic, Autoimmune - immunology; Autoimmune haemolytic anaemia; B lymphocyte; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; CD5; CD5 Antigens - blood; CD5 Antigens - immunology; cd5+ b lymphocyte; Female; Flow Cytometry; Humans; interleukin-10; Interleukin-10 - blood; Interleukin-10 - immunology; Male; Middle Aged; secretory function; Thrombocytopenia - blood; Thrombocytopenia - diagnosis; Thrombocytopenia - immunology; Transforming Growth Factor beta1 - blood; Transforming Growth Factor beta1 - immunology; transforming growth factor β1; Autoimmune haemolytic anaemia; CD5 B lymphocyte; interleukin-10; transforming growth factor β1; secretory function
• ISSN: 0891-6934; 1607-842X
• DOI: 10.1080/08916934.2019.1576644

Objective: To investigate the quantity and secretion function of cytokines-secreted CD5 + B lymphocytes in Autoimmune Haemolytic Anaemia (AIHA)/Evans syndrome (ES) patients. Methods: Twenty-five untreated AIHA/ES patients, 28 remission AIHA/ES patients and 25 healthy controls (HCs) were enrolled in this study. The quantity of CD5 + B lymphocytes which produce interleukin-10 (IL-10) (CD5 + IL-10 + ) and transforming growth factor (TGF-β1) (CD5 + TGF-β1 + ) were detected by flow cytometry (FCM). CD5 + B lymphocytes were sorted from peripheral blood (PB) by FCM and the expression of IL-10 and TGF-β1 mRNA in CD5 + B cells were measured by real-time PCR (RT-PCR). Results: The percentage of CD5 + IL-10 + B cells in CD5 + B lymphocytes in newly diagnosed patients was 82.18 ± 14.78%, which being significantly higher than that of remission AIHA/ES patients (56.68 ± 24.39%) and HCs (51.90 ± 22.95%) (p < .05). The percentage of CD5 + IL-10 + B cells in CD5 + B lymphocytes in newly diagnosed patients was negatively correlated with haemoglobin (Hb), complement 3 (C3) (p < .05) and positively correlated with lactate dehydrogenase (LDH), total bilirubin (TBIL) and indirect unconjugated bilirubin (IBIL) (p < .05). The expression level of IL-10 mRNA in CD5 + B lymphocytes of newly diagnosed patients (49.34 ± 22.84) was higher than that of remission patients (3.97 ± 3.83) and HCs (1.78 ± 1.66) (p < .05). There was no significant difference among three groups with the proportion of CD5 + TGF-β1 + B lymphocytes and the expression level of TGF-β1 mRNA in CD5 + B lymphocytes (p > .05). Conclusions: CD5 + B lymphocytes could secrete IL-10 rather than TGF- β1 which control the immune response in AIHA/ES.