Sensory axons induce epithelial lipid microdomain remodeling and determine the distribution of junctions in the epidermis
Epithelial cell properties are determined by the polarized distribution of membrane lipids, the cytoskeleton, and adhesive junctions. Epithelia are often profusely innervated, but little work has addressed how neurites affect epithelial organization. We previously found that basal keratinocytes in t...
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Published in | Molecular biology of the cell Vol. 34; no. 1; p. ar5 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
The American Society for Cell Biology
01.01.2023
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Series | A Highlights from |
Subjects | |
Online Access | Get full text |
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Summary: | Epithelial cell properties are determined by the polarized distribution of membrane lipids, the cytoskeleton, and adhesive junctions. Epithelia are often profusely innervated, but little work has addressed how neurites affect epithelial organization. We previously found that basal keratinocytes in the zebrafish epidermis enclose axons in ensheathment channels sealed by autotypic junctions. Here we characterized how axons remodel cell membranes, the cytoskeleton, and junctions in basal keratinocytes. At the apical surface of basal keratinocytes, axons organized lipid microdomains quantitatively enriched in reporters for PI(4,5)P2 and liquid-ordered (Lo) membranes. Lipid microdomains supported the formation of cadherin-enriched, F-actin protrusions, which wrapped around axons, likely initiating ensheathment. In the absence of axons, cadherin-enriched microdomains formed on basal cells but did not organize into contiguous domains. Instead, these isolated domains formed heterotypic junctions with periderm cells, a distinct epithelial cell type. Thus, axon endings dramatically remodel polarized epithelial components and regulate epidermal adhesion. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, 415 Curie Blvd., Philadelphia, PA, 19104. Orcid Id: Alvaro Sagasti: 0000-0002-6823-0692 Conflict of interest: The authors declare that they have no conflict of interest. |
ISSN: | 1059-1524 1939-4586 |
DOI: | 10.1091/mbc.E22-09-0396 |