Profiling of Recovery Efficiencies for Three Standard Protocols (FDA-BAM, ISO-11290, and Modified USDA) on Temperature-Injured Listeria monocytogenes

• Published in: Journal of microbiology and biotechnology Vol. 21; no. 9; pp. 954 - 959
• Main Authors: Lee, Hai Yen, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia, Chai, Lay Ching, Universiti Malaysia, Kuala Lumpur, Malaysia, Pui, Chai Fung, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia, Wong, Woan Chwen, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia, Mustafa, Shuhaimi, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia, Cheah, Yoke Kqueen, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia, Issa, Zuraini Mat, Universiti Teknologi MARA Malaysia, Shah Alam, Selangor Darul Ehsan, Malaysia, Nishibuchi, Mitsuaki, Kyoto University, Kyoto, Japan, Radu, Son, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.09.2011; 한국미생물·생명공학회
• Subjects: Biological and medical sciences; Biotechnology; Cold Temperature; Colony Count, Microbial - economics; Colony Count, Microbial - methods; Colony Count, Microbial - standards; Culturing method; Fundamental and applied biological sciences. Psychology; Hot Temperature; LISTERIA MONOCYTOGENES; Listeria monocytogenes - growth & development; Listeria monocytogenes - isolation & purification; Microbial Viability; PROTOCOLE; PROTOCOLOS; PROTOCOLS; REANIMACION; REANIMATION; recovery; RESUSCITATION; semilethal injury; United States; United States Department of Agriculture; United States Food and Drug Administration; 생물학; United States; Temperature; Efficiency; semilethal injury; Injury; Listeria monocytogenes; Bacteria; resuscitation; Method; recovery; Culturing method; protocols; Listerica monocytogenes
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1012.12034

There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures (60℃ and -20℃) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to induce a severe cell injury (up to 100% injury). The injured cells were then subjected to the US Food and Drug Administration (FDA), the ISO-11290, and the modified United States Department of Agriculture (mUSDA) protocols, and plated on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar Listeria for 24 h or 48 h. The evaluation of the total recovery of injured cells was also calculated based on the costs involved in the preparation of media for each protocol. Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze-injured, and heat-freeze-injured cells and was shown to be the most cost effective for heat-freeze-injured cells.