Distribution of antibiotic resistance and the presence of vancomycin-resistance genes (vanA and vanB) in Enterobacteriaceae isolated from the Sea of Marmara, the Canakkale Strait and the Istanbul Strait, Turkey

• Published in: Oceanological and hydrobiological studies Vol. 45; no. 2; pp. 182 - 190
• Main Authors: Çardak, Mine, Altug, Gülçen, Ay, Mustafa, Erol, Ôzlem
• Format: Journal Article
• Language: English
• Published: Berlin De Gruyter 01.06.2016; Walter de Gruyter GmbH
• Subjects: Amoxicillin; Ampicillin; Antibiotic resistance; Antibiotics; Aztreonam; Bacteria; Beta-lactam Antibiotics; Canakkale Strait; Cefotaxime; Ceftazidime; Cefuroxime; Colonies; Disease resistance; Distribution; Domestic water; Drug resistance; E coli; Enterobacteriaceae; Escherichia coli; Genes; Gentamicin; Identification; Istanbul Strait; Kanamycin; Marine; Marine environment; Ofloxacin; PCR; Sea of Marmara; Straits; VanA gene; Vancomycin; Water treatment; Istanbul Turkey; Turkey; Canakkale Strait; MED, Turkey, Istanbul Straits; MED, Marmara Sea; MED, Turkey, Canakkale, Canakkale Strait; MED, Turkey, Istanbul; MED, Turkey
• ISSN: 1897-3191; 1730-413X; 1897-3191
• DOI: 10.1515/ohs-2016-0017

We investigated the frequency of antibiotic resistance of and the presence of vancomycin-resistance genes in samples taken from the Sea of Marmara, and the Istanbul and Canakkale Straits, Turkey. Different colony-forming bacteria were isolated and identified with the VITEK 2 Compact 30 system. The antibiotic resistance of the isolates was determined by the disc diffusion method. The isolates were tested against amoxicillin, ampicillin, aztreonam, ceftazidime, cefotaxime, cefuroxime, ofloxacin, vancomycin, tetracycline, kanamycin and gentamycin. The presence of vancomycin-resistance genes and was also investigated. The level of species was higher in the Sea of Marmara than in the Istanbul Strait and the Canakkale Strait. Isolates showing resistance to the greatest number of antibiotics were identified from isolates. The resistance of the selected bacterial isolates were as follows: kanamycin (82%), vancomycin (78%) and ampicillin (60%). Some intermediately vancomycin-resistant isolates had the gene. This study provides evidence of widespread bacterial resistance to clinically relevant antibiotics in marine environments. It also contributes to the knowledge on the distribution of antibiotic resistance among and indicates the importance of control measures in domestic water treatment.