Rapid pulsed-field gel electrophoresis technique for determination of genetic diversity of Serratia marcescens

• Published in: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Vol. 8; no. 4; pp. 368 - 370
• Main Authors: Ishii, Yoshikazu, Alba, Jimena, Kimura, Soichiro, Yamaguchi, Keizo, Nakashima, Kiyoko, Abe, Yukiko
• Format: Journal Article
• Language: English
• Published: Tokyo Elsevier Ltd 01.12.2002; Springer
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Electrophoresis, Gel, Pulsed-Field - methods; Fundamental and applied biological sciences. Psychology; Genetic Variation; Microbiology; Pulsed-field gel electrophoresis; Rapid method; Serratia marcescens; Serratia marcescens - genetics; Asia; Japan; Rapid method; Pulsed-field gel electrophoresis; Serratia marcescens; Rapid technique; Microbiological investigation; Molecular epidemiology; Bacteria; Genetic diversity; Enterobacteriaceae
• ISSN: 1341-321X; 1437-7780
• DOI: 10.1007/s10156-002-0195-5

Seventeen Serratia marcescens strains were isolated from patients admitted to a hospital in the Tokyo metropolitan area. In order to study their genetic diversity, pulsed-field gel electrophoresis (PFGE) was performed. Clear bands were not observed by the standard PFGE technique following the instruction manual from the company. To obtain clear results, it was necessary to include some additional steps to the preparation of PFGE samples. We added an inactivation step for DNase using formaldehyde, and the bacterial strains were embedded in thinner plug molds than usual. These modifications were effective with all strains, and the complete PFGE pattern was obtained in the samples treated with formaldehyde and using thin plug molds. We established a rapid method to obtain a highquality PFGE result for S. marcescens.