Protective effect of 3-aminobenzamide, an inhibitor of poly (ADP-ribose) synthetase, against streptozotocin-induced diabetes

• Published in: Diabetologia Vol. 28; no. 9; p. 683
• Main Authors: Masiello, P, Cubeddu, T L, Frosina, G, Bergamini, E
• Format: Journal Article
• Language: English
• Published: Germany 01.09.1985
• Subjects: Animals; Benzamides - pharmacology; Blood Glucose - metabolism; Diabetes Mellitus, Experimental - prevention & control; Insulin - metabolism; Insulin Secretion; Islets of Langerhans - drug effects; Islets of Langerhans - metabolism; Male; Niacinamide - pharmacology; Poly(ADP-ribose) Polymerase Inhibitors; Rats; Rats, Inbred Strains; Streptozocin - antagonists & inhibitors; Streptozocin - pharmacology
• ISSN: 0012-186X
• DOI: 10.1007/BF00291976

The addition of 3-aminobenzamide (a potent inhibitor of poly(ADP-ribose)synthetase) into the incubation medium, prevents streptozotocin-induced inhibition of glucose-stimulated insulin release from isolated islets [control 142 +/- 14 microU X islet-1 X h-1; streptozotocin (0.5 mg/ml) 31 +/- 8; 3-aminobenzamide (1.0 mg/ml) 96 +/- 11; streptozotocin plus 3-aminobenzamide 122 +/- 19]. In vivo, intraperitoneal 3-aminobenzamide 300 mg/kg body weight prevents the appearance of overt diabetes in streptozotocin-treated rats. These protective effects of 3-aminobenzamide are dose-dependent and are similar to those exerted by nicotinamide. Taking into account that poly ADP-ribosylation is involved in the repair of damaged DNA, the protection exerted by 3-aminobenzamide against the diabetogenic effect of streptozotocin strongly supports the view that this acute effect may be a major consequence of the activation of DNA repair mechanisms in islet cells.