Evaluation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Conditioning Medium (hWJ-MSCs-CM) or Scorpion Venom Breast Cancer Cell Line In Vitro

• Published in: Journal of gastrointestinal cancer Vol. 53; no. 4; pp. 888 - 901
• Main Authors: Said, Yasmen Mohamed, El-Gamel, Nadia E. A., Ali, Said A., Mohamed, Aly Fahmy
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2022
• Subjects: Cancer Research; Gastroenterology; Internal Medicine; Medicine; Medicine & Public Health; Oncology; Original Research; Radiotherapy; Breast cancer; Wharton’s jelly; Conditioning medium; Mesenchymal stem cells; Apoptosis; Leiurus quinquestriatus
• ISSN: 1941-6628; 1941-6636
• DOI: 10.1007/s12029-021-00762-3

Purpose The present study aimed to evaluate the anticancer potential of Egyptian scorpion Leiurus quinquestriatus venom (ScV) or human Wharton’s jelly-derived mesenchymal stem cells conditioning medium (hWJ-MSCs-CM)/CM against breast cancer (MCF-7) cell line as an alternative effective cancer biotherapy. Methods Venom (ScV) toxicity was performed recording concentration-dependent viability % and ScV IC 50 value was in the order of 100 μg/ml. MCF-7 were treated with hWJ-MSCs-CM used as (25%, 50%, and 75% ml) or the IC 50 of ScV. Apoptotic activity was traced via evaluation the apoptotic (Bax, Casp-3, and Casp-9) and anti-apoptotic genes (Bcl2, ALDOA, and PKM2) profile. Results Both Bax and Casp-3 showed a significant upregulation while anti-apoptotic genes were significantly downregulated. In the meantime, Casp-3 and Casp-9 protein were monitored using ELISA, and their level was less than in control. Additionally, MCF-7 apoptosis was monitored using flow cytometry recording a significant DNA accumulation in the G0–G1 and S phases in case of cell treatment with ScV or CM75% ml and 50% ml. Also, there was a significant total necrotic cells % compared with control cells, and total apoptosis under the effect of ScV or CM75% ml was significantly elevated than rest of treatment. Conclusion Apoptosis induction was both dose- and time-dependent for hWJ-MSCs-CM and ScV. According to the present study and other studies, there is an ample evidence that hWJ-MSCs-CM and the venom IC 50 abolish tumor growth.