Bottlebrush Glycopolymers from 2-Oxazolines and Acrylamides for Targeting Dendritic Cell-Specific Intercellular Adhesion Molecule-3-Grabbing Nonintegrin and Mannose-Binding Lectin

• Published in: Biomacromolecules Vol. 21; no. 6; pp. 2298 - 2308
• Main Authors: Beyer, Valentin P, Monaco, Alessandra, Napier, Richard, Yilmaz, Gokhan, Becer, C Remzi
• Format: Journal Article
• Language: English
• Published: United States 08.06.2020
• Subjects: Acrylamides; Animals; Cell Adhesion Molecules; Chick Embryo; Intercellular Adhesion Molecule-3; Lectins, C-Type; Oxazoles; Receptors, Cell Surface
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/acs.biomac.0c00246

Lectins are omnipresent carbohydrate binding proteins that are involved in a multitude of biological processes. Unearthing their binding properties is a powerful tool toward the understanding and modification of their functions in biological applications. Herein, we present the synthesis of glycopolymers with a brush architecture via a "grafting from" methodology. The use of a versatile 2-oxazoline inimer was demonstrated to open avenues for a wide range of 2-oxazoline/acrylamide bottle brush polymers utilizing aqueous Cu-mediated reversible deactivation radical polymerization (Cu-RDRP). The polymers in the obtained library were assessed for their thermal properties in aqueous solution and their binding toward the C-type animal lectins dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) and mannose-binding lectin (MBL) via surface plasmon resonance spectrometry. The encapsulation properties of a hydrophobic drug-mimicking compound demonstrated the potential use of glyco brush copolymers in biological applications.