Sublingual immunisation with GBS serotype III capsular polysaccharide-tetanus toxoid conjugate vaccine induces systemic and mucosal antibody responses which are opsonophagocytic and inhibit GBS colonisation of vaginal epithelial cells

• Published in: Vaccine Vol. 40; no. 42; pp. 6055 - 6063
• Main Authors: Deifallah Yousif, Mohamed, Felek, Arif, Saydam, Manolya, Wilson, Seanette, Murdan, Sudaxshina, Mawas, Fatme
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 06.10.2022; Elsevier Limited
• Subjects: Antibiotics; Antibodies; Antibody response; Antisera; Babies; Capsular polysaccharides; Cholera; Clinical trials; Colonization; Conjugate vaccine; Conjugates; Disease; Epithelial cells; Epithelium; Ethnicity; Fetuses; Group B streptococcus; Homology; Immunization; Immunoglobulin A; Immunoglobulin G; Infections; Intestine; Meningitis; Morbidity; Mothers; Mucosa; Mucosal immunity; Opsonophagocytosis; Polysaccharides; Proteins; Stillbirth; Streptococcus infections; Sublingual; Tetanus; Vaccines; Vagina; Vaginal colonisation; Womens health; United Kingdom > UK; Africa; Mucosal immunity; Vaginal colonisation; Sublingual; Conjugate vaccine; Group B streptococcus; Opsonophagocytosis
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2022.08.064

No vaccines are currently licensed against Group B streptococcus (GBS), an important cause of morbidity and mortality in babies and adults. Using a mouse model, and in vitro opsonophagocytosis and colonisation assays, we evaluated the potential of a sublingually-administered polysaccharide-conjugate vaccine against GBS serotype III. Sublingual immunisation of mice with 10 µg of GBS conjugate vaccine once a week for 5 weeks induced a substantial systemic IgG anti-polysaccharide response which was similar to the level induced by subcutaneous immunsation. In addition, sublingual immunisation also induced mucosal (IgA) antibody responses in the mouth, intestines and vagina. Immune sera and intestinal washes were functionally active at mediating killing of the homologous GBS serotype III in an opsonophagocytosis assay. In addition, intestinal and vaginal washes inhibited the colonisation of mouse vaginal epithelial cells by the vaccine homologous strain. These results suggest that, in addition to the induction of high levels of IgG antibodies that could be transduced from the immunised mother to the foetus to protect the newborn against GBS infection, sublingual immunisation can elicit a substantial mucosal antibody response which might play an important role in the prevention of GBS colonisation in immunised women, thereby eliminating the risk of GBS transmission from the mother to the baby during pregnancy or at birth.