HSLC_FUGE: HIGH SPEED AND LOW COST LABORATORY CENTRIFUGE FOR GENOMIC DNA PURIFICATION

A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity. Like all other centrifuges, laboratory centrifuges work by the sedimentation principle, wher...

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Bibliographic Details
Published inJournal of mechanics in medicine and biology Vol. 12; no. 5; pp. 1240026 - 1240012
Main Authors MABROUK, MAI S., EZZ, MOHAMED A.
Format Journal Article
LanguageEnglish
Published Singapore World Scientific Publishing Company 01.12.2012
World Scientific Publishing Co. Pte., Ltd
Subjects
Centrifuges
Chemical synthesis
Deoxyribonucleic acid
DNA
High speed
Laboratories
Laboratory equipment
Muscles
Polymorphism
Sedimentation
Centrifuge
genomics
DNA extraction
DNA purification
high speed
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Summary:A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity. Like all other centrifuges, laboratory centrifuges work by the sedimentation principle, where the centripetal acceleration is used to separate substances of greater and lesser density. The information for synthesizing the molecules that allow organisms to survive and replicate is encoded in genomic DNA. Extracted and purified genomic DNA is very important for the analysis of single nucleotide polymorphisms (SNPs), disease states, and for many other multiplex and real-time PCR applications. The aim of this work is to design a genomic DNA extraction system that satisfies downstream application needs necessary for the successful completion of experiments and able to isolate a purified genomic DNA from many sources from bacteria to humans and also is able to encompass tissues from blood to muscle and from leaf to seed. Results revealed that the proposed system works with high efficiency and spins at up to 12,000 RPM to facilitate separation of the different phases of the extraction.
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ISSN:0219-5194
1793-6810
DOI:10.1142/S021951941240026X