Involvement of the JAK‐STAT pathway in the molecular landscape of tyrosine kinase fusion‐negative hypereosinophilic syndromes: A nationwide CEREO study

• Published in: American journal of hematology Vol. 99; no. 6; pp. 1108 - 1118
• Main Authors: Groh, Matthieu, Fenwarth, Laurène, Labro, Mathilde, Boudry, Augustin, Fournier, Elise, Wemeau, Mathieu, Marceau‐Renaut, Alice, Daltro de Oliveira, Rafael, Abraham, Julie, Barry, Marly, Blanche, Philippe, Bodard, Quentin, Braun, Thorsten, Chebrek, Safia, Decamp, Matthieu, Durel, Cécile‐Audrey, Forcade, Edouard, Gerfaud‐Valentin, Mathieu, Golfier, Camille, Gourguechon, Clément, Grardel, Nathalie, Kosmider, Olivier, Martis, Nihal, Melboucy Belkhir, Sarah, Merabet, Fatiha, Michon, Adrien, Moreau, Stéphane, Morice, Cécile, Néel, Antoine, Nicolini, Franck E., Pascal, Laurent, Pasquier, Florence, Pieragostini, Andrea, Roche‐Lestienne, Catherine, Rousselot, Philippe, Terriou, Louis, Thiebaut‐Bertrand, Anne, Viallard, Jean‐François, Preudhomme, Claude, Kahn, Jean‐Emmanuel, Lefevre, Guillaume, Duployez, Nicolas
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.06.2024; Wiley Subscription Services, Inc; Wiley
• Subjects: Adult; Aged; Aged, 80 and over; Chromatin; Corticosteroids; Eosinophilia; Female; Genes; Humans; Hypereosinophilic Syndrome - drug therapy; Hypereosinophilic Syndrome - genetics; Janus kinase; Janus Kinase 1 - genetics; Janus kinase 2; Janus Kinase 2 - genetics; Kinases; Leukocytes (eosinophilic); Life Sciences; Male; Medical prognosis; Middle Aged; Multivariate analysis; Mutation; Myelodysplastic syndrome; Polycythemia; Pyrimidines - therapeutic use; Signal Transduction; STAT5 Transcription Factor - genetics; Young Adult
• ISSN: 0361-8609; 1096-8652; 1096-8652
• DOI: 10.1002/ajh.27306

We investigated using a custom NGS panel of 149 genes the mutational landscape of 64 consecutive adult patients with tyrosine kinase fusion‐negative hypereosinophilia (HE)/hypereosinophilic syndrome (HES) harboring features suggestive of myeloid neoplasm. At least one mutation was reported in 50/64 (78%) patients (compared to 8/44 (18%) patients with idiopathic HE/HES/HEUS used as controls; p < .001). Thirty‐five patients (54%) had at least one mutation involving the JAK‐STAT pathway, including STAT5B (n = 18, among which the hotspot N642H, n = 13), JAK1 (indels in exon 13, n = 5; V658F/L, n = 2), and JAK2 (V617F, n = 6; indels in exon 13, n = 2). Other previously undescribed somatic mutations were also found in JAK2, JAK1, STAT5B, and STAT5A, including three patients who shared the same STAT5A V707fs mutation and features consistent with primary polycythemia. Nearly all JAK‐STAT mutations were preceded by (or associated with) myelodysplasia‐related gene mutations, especially in RNA‐splicing genes or chromatin modifiers. In multivariate analysis, neurologic involvement (hazard ratio [HR] 4.95 [1.87–13.13]; p = .001), anemia (HR 5.50 [2.24–13.49]; p < .001), and the presence of a high‐risk mutation (as per the molecular international prognosis scoring system: HR 6.87 [2.39–19.72]; p < .001) were independently associated with impaired overall survival. While corticosteroids were ineffective in all treated JAK‐STAT‐mutated patients, ruxolitinib showed positive hematological responses including in STAT5A‐mutated patients. These findings emphasize the usefulness of NGS for the workup of tyrosine kinase fusion‐negative HE/HES patients and support the use of JAK inhibitors in this setting. Updated classifications could consider patients with JAK‐STAT mutations and eosinophilia as a new “gene mutated‐entity” that could be differentiated from CEL, NOS, and idiopathic HES. Mutations of the JAK‐STAT pathway (JAK1, JAK2, STAT5B, STAT5A) are frequent (>50% of patients) in patients with tyrosine kinase fusion‐negative hypereosinophilic syndromes, and are amenable to JAK inhibition.