Posttranslational Inhibition of Ty1 Retrotransposition by Nucleotide Excision Repair/Transcription Factor TFIIH Subunits Ssl2p and Rad3p

• Published in: Genetics (Austin) Vol. 148; no. 4; pp. 1743 - 1761
• Main Authors: Lee, Bum-Soo, Lichtenstein, Conrad P, Faiola, Brenda, Rinckel, Lori A, Wysock, William, Curcio, M. Joan, Garfinkel, David J
• Format: Journal Article
• Language: English
• Published: United States Genetics Soc America 01.04.1998
• Subjects: Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - metabolism; Alcohol Oxidoreductases; Alleles; Aminohydrolases; Chromosomes, Fungal; DNA Helicases - genetics; DNA Helicases - metabolism; DNA Repair; DNA Transposable Elements; DNA, Complementary; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression Regulation; Mutagenesis; Protein Biosynthesis; Pyrophosphatases; Recombination, Genetic; Retroelements; Saccharomyces cerevisiae Proteins; TATA-Binding Protein Associated Factors; Transcription Factor TFIID; Transcription Factor TFIIH; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription Factors, TFII
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/148.4.1743

rtt4-1 (regulator of Ty transposition) is a cellular mutation that permits a high level of spontaneous Ty1 retrotransposition in Saccharomyces cerevisiae. The RTT4 gene is allelic with SSL2 (RAD25), which encodes a DNA helicase present in basal transcription (TFIIH) and nucleotide excision repair (NER) complexes. The ssl2-rtt (rtt4-1) mutation stimulates Ty1 retrotransposition, but does not alter Ty1 target site preferences, or increase cDNA or mitotic recombination. In addition to ssl2-rtt, the ssl2-dead and SSL2-1 mutations stimulate Ty1 transposition without altering the level of Ty1 RNA or proteins. However, the level of Ty1 cDNA markedly increases in the ssl2 mutants. Like SSL2, certain mutations in another NER/TFIIH DNA helicase encoded by RAD3 stimulate Ty1 transposition. Although Ssl2p and Rad3p are required for NER, inhibition of Ty1 transposition is independent of Ssl2p and Rad3p NER functions. Our work suggests that NER/TFIIH subunits antagonize Ty1 transposition posttranslationally by inhibiting reverse transcription or destabilizing Ty1 cDNA.