Pathogenic interactions between variants of cauliflower mosaic virus and Arabidopsis thaliana

• Published in: Journal of experimental botany Vol. 49; no. 321; pp. 731 - 737
• Main Authors: Cecchini, Edi, Al-Kaff, Nadia S., Bannister, Andrew, Giannakou, Maria E., McCallum, Don G., Maule, Andrew J., Milner, Joel J., Covey, Simon N.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.04.1998; OXFORD UNIVERSITY PRESS
• Subjects: Biological and medical sciences; Caulimovirus; Cruciferae; Fundamental and applied biological sciences. Psychology; Growth retardation; Infections; Necrosis; Pathology. Damages, economic importance; Phenotypes; Phytopathology. Animal pests. Plant and forest protection; plant mutants; Plant veins; Plant viruses and viroids; Plants; Plants and the Environment; symptom expression; Symptoms; Turnips; Viruses; Cauliflower mosaic virus; Pathogenesis; Genetic variant; Gene expression; Genetic determinism; Host virus relation; Virus; Arabidopsis thaliana; Caulimovirus; Cruciferae; Dicotyledones; Angiospermae; Spermatophyta; Mutation; Experimental plant
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/49.321.731

Pathogenic interactions between genetic variants of cauliflower mosaic virus (CaMV) and Arabidopsis thaliana were characterized to identify combinations potentially useful in molecular genetic analysis. Infections of a glabrous mutant (gl1) of Arabidopsis ecotype Columbia (Col-O gl1) by 30 CaMV isolates were assessed by recording symptom character. Thirteen isolates failed to cause symptoms; the remainder induced symptoms that varied between mild and very severe. Some CaMV isolates produced symptoms in Arabidopsis that differed significantly in severity or character from those produced in a standard host Brassica rapa (turnip). A greater variety of symptom types was observed in a single Arabidopsis ecotype infected with a range of CaMV isolates than was found in a range of Arabidopsis ecotypes infected with a single, typical CaMV isolate (Cabb B-JI). One isolate, Bari-1, that was asymptomatic but accumulated virus in Arabidopsis ecotype Col-O gl1, caused mild symptoms in ecotype Ler gl1. A hybrid virus constructed from CaMV isolates Cabb B-JI and Bari-1 produced symptoms in Arabidopsis variants that were more severe than in either parental isolate. From a screen of EMS-mutagenized Arabidopsis, one mutant (Col-O dv1) with a pale-green, dark-vein phenotype which had an altered symptom response to CaMV, was isolated. From this, a phenotypically near-normal revertant (Col-O dv1R) spontaneously arose, but which showed altered responses to CaMV. Infection of Col-O dv1R by CaMV isolate Bari-1 elicited symptoms unlike the parent Arabidopsis ecotype (Col-O gl1). Also, Col-O dv1 and Col-O dv1R expressed an uncharacteristic necrotic reaction to CaMV.