Organic disulfides as a means to generate streak-free two-dimensional maps with narrow range basic immobilized pH gradient strips as first dimension

• Published in: Proteomics (Weinheim) Vol. 2; no. 11; pp. 1630 - 1632
• Main Authors: Olsson, Ingmar, Larsson, Kjell, Palmgren, Ronnie, Bjellqvist, Bengt
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.11.2002; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Animals; Biological and medical sciences; Biotechnology; Disulfides - chemistry; Electrophoresis, Gel, Two-Dimensional - methods; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Immobilization of enzymes and other molecules; Immobilization techniques; Liver - chemistry; Methods. Procedures. Technologies; Mice; Miscellaneous; Molecular and cellular biology; Molecular genetics; Proteome - analysis; Proteome - chemistry; Proteomics - methods; Two-dimensional electrophoresis; pH gradient; Proteomics; Proteome; Two dimensional electrophoresis; Method; Molecular biology; Protein
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/1615-9861(200211)2:11<1630::AID-PROT1630>3.0.CO;2-N

Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two‐dimensional (2‐D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak™). Use of DeStreak also results in 2‐D maps with simplified spot patterns and improved reproducibility.