Development of a model system to assess the impact of genetically modified corn and aubergine plants on arbuscular mycorrhizal fungi

• Published in: Plant and soil Vol. 266; no. 1-2; pp. 69 - 75
• Main Authors: Turrini, Alessandra, Sbrana, Cristiana, Nuti, Marco Paolo, Pietrangeli, Bianca Maria, Giovannetti, Manuela
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 2005
• Subjects: Appressoria; arbuscular mycorrhizas; Corn; Defensins; Exudates; Fungi; Genetically altered foods; Genetically modified organisms; Host plants; Infection; Life cycle; Microorganisms; Morphogenesis; Mycelia; Nutrition; Plant nutrition; Roots; Soil fertility; Soil microorganisms; Toxins; Transgenic plants
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-005-4892-6

We developed an experimental model system to monitor the impact of generically modified (GM) plants on arbuscular mycorrhizal (AM) fungi, a group of non-target soil microorganisms, fundamental for soil fertility and plant nutrition. The system allowed us to study the effects of root exudates of both commercial Bt corn and aubergine plants expressing Dm-AMP1 defensin on different stages of the life cycle of the AM fungal species G. mosseae. Root exudates of Bt 176 corn significantly reduced pre-symbiotic hyphal growth, compared to Bt 11 and non-transgenic plants. No differences were found in mycelial growth in the presence of Dm-AMP1 and control plant root exudates. Differential hyphal morphogenesis occurred irrespective of the plant line, suggesting that both exuded Bt toxin and defensin do not interfere with fungal host recognition mechanisms. Bt 176 affected the regular development of appressoria, 36% of which failed to produce viable infection pegs. Our experimental model system represents an easy assay for testing the impact of GM plants on non-target soil-borne AM fungi.