A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

• Published in: Journal of visualized experiments no. 108; p. 53791
• Main Authors: Gao, Siyue, Glasser, Jessica, He, Lili
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 19.02.2016
• Subjects: Biological Assay; Chemistry; Metal Nanoparticles - chemistry; Silver Nitrate - analysis; Spectrum Analysis, Raman - methods
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/53791

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.