Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis

• Published in: Analytical biochemistry Vol. 325; no. 2; pp. 255 - 259
• Main Authors: Zhang, Xinfeng, Ma, Yue, Liu, Hong, de Sa, Paula F.G, Brown, Phyllis R, Dain, Joel A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.02.2004
• Subjects: AGE; Capillary electrophoresis; Electrophoresis, Capillary - methods; Electrophoresis, Polyacrylamide Gel; Fibrinogen; Fibrinogen - metabolism; Glucosamine; Glucosamine - metabolism; Glycation End Products, Advanced - analysis; Glycation End Products, Advanced - metabolism; Human serum albumin; Humans; Maillard Reaction; MRP; Serum Albumin - metabolism; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Human serum albumin; Glucosamine; MRP; Capillary electrophoresis; Fibrinogen; AGE
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2003.11.001

Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel electrophoresis demonstrated the generation of high-molecular-weight fibrinogen and HSA MRP by GlcN. A simple and rapid capillary electrophoresis method was developed to separate MRP formed by the reaction of GlcN with proteins from GlcN autocondensation products.