In vivo studies of the biosynthesis of alpha-eleostearic acid in the seed of Momordica charantia L

• Published in: Plant physiology (Bethesda) Vol. 113; no. 4; pp. 1343 - 1349
• Main Authors: Liu, L. (Iowa State University, Ames, IA.), Hammond, E.G, Nikolau, B.J
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.04.1997
• Subjects: ACETATE; ACETATES; ACETATOS; ACIDE LINOLEIQUE; ACIDE LINOLENIQUE; ACIDE OLEIQUE; ACIDE STEARIQUE; ACIDO ESTEARICO; ACIDO LINOLEICO; ACIDO LINOLENICO; ACIDO OLEICO; Agronomy. Soil science and plant productions; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; Biochemistry and Enzymology; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; CHEMICAL COMPOSITION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Conjugated linoleic acids; CRECIMIENTO; CROISSANCE; DERIVATIVES; Economic plant physiology; Esters; Fatty acids; Fundamental and applied biological sciences. Psychology; GRAINE; GROWTH; ISOTOPE RADIOACTIF; KINETICS; LINOLEIC ACID; LINOLENIC ACID; LIPIDE; LIPIDOS; LIPIDS; METABOLISM; Metabolism. Physicochemical requirements; METABOLISME; METABOLISMO; MOMORDICA CHARANTIA; Nitrogen metabolism and other ones (excepting carbon metabolism); Nutrition. Photosynthesis. Respiration. Metabolism; OLEIC ACID; PESO; Plant physiology and development; Plants; POIDS; PRECURSORS; QUANTITATIVE ANALYSIS; Radioactive decay; RADIOACTIVE TRACERS; RADIOISOTOPES; RADIOISOTOPOS; SEED; SEEDS; SEMENCE; SEMILLA; SEMILLAS; Solvents; STEARIC ACID; WEIGHT; Radiolabelling; Cucurbitaceae; Polyunsaturated fatty acid; Lipids; Biosynthesis; Acetate; Metabolism; Linoleic acid; Linolenic acid; Vegetable crop; Oleic acid; Dicotyledones; Seed; Angiospermae; Biosynthesis precursor; Spermatophyta; Kinetics
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.113.4.1343

In vivo radiotracer experiments using 14C-labeled acetate, oleate, linoleate, and linolenate were conducted to investigate the biosynthesis of alpha-eleostearic acid in the seeds of Momordica charantia. With the exception of [14C]linolenate, all of these precursors radioactively labeled alpha-eleostearate. Kinetics of the time course of metabolism of the radioactive precursors indicate that linoleate is the acyl precursor of alpha-eleostearate and that its conversion to alpha-eleostearate occurs while the acyl moiety is esterified to PC. Pulse-chase experiments with 14C-labeled acetate or linoleate provided additional corroborative evidence that linoleoyl PC is the precursor of alpha-eleostearoyl PC