Osteogenic properties and anti-osteoporosis activity of calcium hydroxyapatite from Katsuwonus pelamis bone and its water-soluble forms

• Published in: Fisheries science Vol. 89; no. 6; pp. 837 - 852
• Main Authors: Aenglong, Chakkapat, Tang, Qing-Juan, Tanasawet, Supita, Klaypradit, Wanwimol, Sukketsiri, Wanida
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01.11.2023; Springer Nature B.V
• Subjects: Alizarin; Alkaline phosphatase; Biomedical and Life Sciences; Biomedical materials; Calcium; Cell differentiation; Cell proliferation; Cytotoxicity; Dexamethasone; Differentiation; Fish & Wildlife Biology & Management; Food Science; Freshwater & Marine Ecology; Glucocorticoids; Hydroxides; Hydroxyapatite; Katsuwonus pelamis; Lactate; Life Sciences; Monomolecular films; Nucleotide sequence; Original Article; Osteoblastogenesis; Osteoblasts; Osteoporosis; Phosphatase; Proliferation; Water chemistry; Structured hydroxyapatite; Osteoporosis; Hydroxyapatite; Osteogenic; Osteoblasts
• ISSN: 0919-9268; 1444-2906
• DOI: 10.1007/s12562-023-01711-8

In this study, the osteogenic properties and anti-osteoporosis activity of calcium hydroxyapatite (HA) obtained from Katsuwonus pelamis tuna bone and water-soluble structured forms of HA, such as the structured HA chloride citrate compound (HA-Cl-Ci) and the structured HA hydroxide lactate compound (HA-OH-Lac), were investigated. There was no significant cytotoxicity towards MC3T3-E1 pre-osteoblast and Caco-2 cells. HA enhanced osteogenic proliferation and differentiation after 7, 14, and 21 days of treatment, as demonstrated by alizarin red staining, alkaline phosphatase (ALP) activity, and qRT-PCR. Also, the anti-osteoporosis efficiencies of HA, HA-Cl-Ci, and HA-OH-Lac were evaluated in dexamethasone-induced MC3T3-E1 osteoblasts. HA, HA-Cl-Ci, and HA-OH-Lac had the potential to prevent glucocorticoid-induced osteoporosis effects. In addition, the transcellular transport of these compounds across the Caco-2 cell monolayers was investigated. Ca, P, and Mg derived from HA, HA-Cl-Ci, and HA-OH-Lac were absorbed in the intestinal tract. The absorption of these minerals may have resulted in the osteogenic proliferation, differentiation, and anti-osteoporosis activity.