An integrated bioprocess for xylanase production from agriculture waste under open non-sterilized conditions: Biofabrication as fermentation tool

• Published in: Journal of cleaner production Vol. 193; pp. 194 - 205
• Main Authors: Simair, Altaf Ahmed, Qureshi, Abdul Sattar, Simair, Sippy Pirah, Khushk, Imrana, Klykov, Sergey P., Ali, Chaudhry Haider, Lu, Changrui
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 20.08.2018
• Subjects: Bacillus cereus TH-050; Lignocellulosic biomass; Mathematical modeling; Open fermentation; Saccharification; Solid substrate fermentation; Bacillus cereus TH-050; Saccharification; Solid substrate fermentation; Open fermentation; Mathematical modeling; Lignocellulosic biomass
• ISSN: 0959-6526; 1879-1786
• DOI: 10.1016/j.jclepro.2018.05.020

Indiscriminate disposal of the solid waste generated from various agricultural practices cause detrimental effects on the environment. Utilization of the waste biomass for production of value-added products through biotechnological intervention not only helps to combat environmental pollution but also contributes significantly to the economy. In this study, a biofabrication software (fermentor tool) was applied to analyze statistics of xylanase production under solid non-sterile fermentation conditions. In solid substrate fermentation wheat bran, banana peel, millet waste, and sugarcane bagasse were evaluated. Maximal xylanase titer of 10545 U/g dry matter was obtained when wheat bran was used as a substrate (4 days incubation at 50 °C and pH value of 8.0). Partially purified xylanase was applied in saccharification of lignocellulosic materials to assess its potential application in a lignocellulosic biorefinery. In this context, the precision of predicted mathematical model was evaluated and fermentation provided a good prediction of the concentration of cell, substrate and product. Second, open non-sterilized fermentation method was established to save sterilization cost that seems to be a very economical and practically applicable to the enzyme industry. [Display omitted] •Thermoalkalophilic strain with high xylanase production rate was obtained.•Open non-sterile fermentation for xylanase production was developed.•Agricultural solid waste was utilized for fermentable sugar production.•Xylanase proved potential for saccharification of various lignocellulosic biomass.•Biofabrication software was applied for mathematical model-based optimization.