SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines

Short interspersed elements (SINEs), ubiquitous components of eukaryote genomes, can provide primers to PCR amplify DNA segments flanked by these sequences. Species (or family) specificity of SINEs has turned out to be valuable for isolation of species-specific sequences from complex and limited DNA...

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Bibliographic Details
Published inMammalian genome Vol. 8; no. 5; pp. 365 - 367
Main Authors Kostia, S, Vilkki, J, Pirinen, M, Womack, J E, Barendse, W, Varvio, S L
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.05.1997
Subjects
Animals
Base Sequence
Cattle - genetics
Cell lines
Chromosome Mapping
DNA - genetics
DNA Primers - genetics
Gene Targeting - methods
Hybrid Cells
Microsatellite Repeats
Molecular Sequence Data
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Summary:Short interspersed elements (SINEs), ubiquitous components of eukaryote genomes, can provide primers to PCR amplify DNA segments flanked by these sequences. Species (or family) specificity of SINEs has turned out to be valuable for isolation of species-specific sequences from complex and limited DNA sources by inter-SINE PCR amplification. A high frequency of bovine C-A SINE-elements is known to possess dinucleotide microsatellite tails, suggesting their enrichment among sequences amplified by inter-SINE PCR. In the present paper we describe a SINE-targeting method utilizing this association in microsatellite marker isolation from specific bovine chromosomes. This method may serve as a tool to increase marker density in regions of interest, such as sites of putative QTL, or gaps in the available bovine linkage maps.
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ISSN:0938-8990
1432-1777
DOI:10.1007/s003359900443