Similarity in the acute cytotoxic response of mammalian cells to mercury (II) and X-rays: DNA damage and glutathione depletion

Pronounced strand breakage of DNA analyzed by alkaline elution techniques was produced in intact Chinese hamster ovary cells by 25 μM HgCl2 within 1 hr or 100 μM HgCl2 within 15 min. HgCl2-induced strand breakage was directly proportional to concentration up to 100 μM and to time within 1 hr. Levels...

Full description

Saved in:
Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 108; no. 2; pp. 614 - 619
Main Authors Cantoni, Orazio, Evans, R. Mark, Costa, Max
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 30.09.1982
Subjects
Animals
Cells, Cultured
Cricetinae
Cricetulus
DNA - metabolism
DNA - radiation effects
DNA, Single-Stranded - metabolism
environmental degradation
Female
Glutathione - metabolism
Mercury - toxicity
Ovary
pollution
waste management
GSH
SSF
CHO
Online AccessGet full text

Cover

More Information
Summary:Pronounced strand breakage of DNA analyzed by alkaline elution techniques was produced in intact Chinese hamster ovary cells by 25 μM HgCl2 within 1 hr or 100 μM HgCl2 within 15 min. HgCl2-induced strand breakage was directly proportional to concentration up to 100 μM and to time within 1 hr. Levels of reduced glutathione decreased following HgCl2 in parallel with the induction of DNA strand breakage. Evidence is presented that this rapid and pronounced induction of DNA strand breaks and other cytotoxic responses following acute exposure to HgCl2 resembles the cellular effects of X-rays.
Bibliography:T
T00
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(82)90873-7